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mouse anti ubx  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti ubx
    Mouse Anti Ubx, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+ubx/anti-floor+plate+marker/bio_rxiv__64898__2026__03__17__712157-167-49-57
    Average 94 stars, based on 54 article reviews
    mouse anti ubx - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: Reduced histone gene copy number disrupts Drosophila Polycomb function.
    Article Snippet: The chromatin of animal cells contains two types of histones: canonical histones that are expressed during S phase of the cell cycle to package the newly replicated genome, and variant histones with specialized functions that are expressed throughout the cell cycle and in nonproliferating cells.. Determining whether and how canonical and variant histones cooperate to regulate genome function is integral to understanding how chromatin-based processes affect normal and pathological development.. Here, we demonstrate that variant histone H3.3 is essential for Drosophila development only when canonical histone gene copy number is reduced, suggesting that coordination between canonical H3.2 and variant H3.3 expression is necessary to provide sufficient H3 protein for normal genome function.

    Immunofluorescence:

    Article Title: Interallelic cis-regulatory dominance promotes robustness and evolutionary innovation
    Article Snippet: .. HCR was combined with immunofluorescence , where after the postfix step at the end of the HCR protocol, the embryos were washed with SSCT (5xSSC, 0.1% Tween20), incubated for 25 minutes with SSCT blocking solution (Roche Western Blocking Reagent #11921673001; diluted 1:5 in SSCT), and then incubated overnight with mouse anti-Ubx (1:20 in SSCT blocking solution, FP3.38-c, DSHB, supernatant) at 4°C. .. For the immunofluorescence-only conditions, images were acquired on a Zeiss LSM880 confocal microscope using the semi-automated adaptive feedback 3D microscopy pipeline .

    Incubation:

    Article Title: Interallelic cis-regulatory dominance promotes robustness and evolutionary innovation
    Article Snippet: .. HCR was combined with immunofluorescence , where after the postfix step at the end of the HCR protocol, the embryos were washed with SSCT (5xSSC, 0.1% Tween20), incubated for 25 minutes with SSCT blocking solution (Roche Western Blocking Reagent #11921673001; diluted 1:5 in SSCT), and then incubated overnight with mouse anti-Ubx (1:20 in SSCT blocking solution, FP3.38-c, DSHB, supernatant) at 4°C. .. For the immunofluorescence-only conditions, images were acquired on a Zeiss LSM880 confocal microscope using the semi-automated adaptive feedback 3D microscopy pipeline .

    Blocking Assay:

    Article Title: Interallelic cis-regulatory dominance promotes robustness and evolutionary innovation
    Article Snippet: .. HCR was combined with immunofluorescence , where after the postfix step at the end of the HCR protocol, the embryos were washed with SSCT (5xSSC, 0.1% Tween20), incubated for 25 minutes with SSCT blocking solution (Roche Western Blocking Reagent #11921673001; diluted 1:5 in SSCT), and then incubated overnight with mouse anti-Ubx (1:20 in SSCT blocking solution, FP3.38-c, DSHB, supernatant) at 4°C. .. For the immunofluorescence-only conditions, images were acquired on a Zeiss LSM880 confocal microscope using the semi-automated adaptive feedback 3D microscopy pipeline .

    Western Blot:

    Article Title: Interallelic cis-regulatory dominance promotes robustness and evolutionary innovation
    Article Snippet: .. HCR was combined with immunofluorescence , where after the postfix step at the end of the HCR protocol, the embryos were washed with SSCT (5xSSC, 0.1% Tween20), incubated for 25 minutes with SSCT blocking solution (Roche Western Blocking Reagent #11921673001; diluted 1:5 in SSCT), and then incubated overnight with mouse anti-Ubx (1:20 in SSCT blocking solution, FP3.38-c, DSHB, supernatant) at 4°C. .. For the immunofluorescence-only conditions, images were acquired on a Zeiss LSM880 confocal microscope using the semi-automated adaptive feedback 3D microscopy pipeline .



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